Promega Corporation

PowerPlex®16 BIO System Technical Manual

Literature # TMD016

The PowerPlex® 16 BIO System allows the coamplification and three-color detection of sixteen loci (fifteen STR loci and Amelogenin). The PowerPlex® 16 BIO System contains the loci Penta E, D18S51, D21S11, TH01, D3S1358, FGA, TPOX, D8S1179, vWA, Amelogenin, Penta D, CSF1PO, D16S539, D7S820, D13S317 and D5S818. In this system, one primer specific for Penta E, D18S51, D21S11, TH01 and D3S1358 is labeled with fluorescein (FL); one primer specific for FGA, TPOX, D8S1179, vWA and Amelogenin is labeled with Rhodamine Red™-X; and one primer specific for Penta D, CSF1PO, D16S539, D7S820, D13S317 and D5S818 is labeled with 6-carboxy-4′,5′-dichloro-2′,7′-dimethoxy-fluorescein(JOE). All sixteen loci are amplified simultaneously in a single tube and analyzed in a single gel lane.

Printed in USA. Revised 5/12.

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