TFIIB, Human, Recombinant

rhTFIIB is a general transcription factor involved in formation of an active complex in vitro capable of specifically initiating RNA synthesis by RNA polymerase II. An early stage of initiation complex assembly involves the formation of a D-B or D-A-B complex, which consists of TFIID, TFIIB (TFIIA) and the promoter DNA. The stability of the D-B and D-A-B complexes is thought to be greater than that of TFIID and DNA alone. The full-length human cDNA for TFIIB is expressed in E. coli and has a molecular weight of 32kDa. TFIIB alone does not have DNA-binding activity.

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rhTFIIB is a general transcription factor involved in formation of an active complex in vitro capable of specifically initiating RNA synthesis by RNA polymerase II. An early stage of initiation complex assembly involves the formation of a D-B or D-A-B complex, which consists of TFIID, TFIIB (TFIIA) and the promoter DNA. The stability of the D-B and D-A-B complexes is thought to be greater than that of TFIID and DNA alone. The full-length human cDNA for TFIIB is expressed in E. coli and has a molecular weight of 32kDa. TFIIB alone does not have DNA-binding activity.

Features - Benefits

• Performance-Tested: Tested by gel shift assay for the formation of the D-B complex. Tested for in vitro transcriptional activity.

Applications

• Gel shift assays.
• Transcriptional activation in vitro.

Notes

1gsu = amount of protein required to gel shift the TFIID oligonucleotide in the presence of rhTFIID under defined conditions.

References

1. Ha, I. et al. (1991) Nature 352, 689–95.
2. Maldonado, E. et al. (1990) Mol. Cell Biol. 10, 6335–47.

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