Riboprobe® Combination Systems

The Riboprobe® Combination Systems are designed for in vitro preparation of high-specific-activity single-stranded RNA probes or microgram quantities of defined RNA transcripts from cloned DNA inserts. The Riboprobe® Combination Systems include the RNA polymerases, all of the required reagents (excluding radioisotope) for performing transcription reactions in vitro and RQ1 RNase-Free DNase (Cat.# M6101) for removal of the template following transcription.

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The Riboprobe® Combination Systems are designed for in vitro preparation of high-specific-activity single-stranded RNA probes or microgram quantities of defined RNA transcripts from cloned DNA inserts. The Riboprobe® Combination Systems include the RNA polymerases, all of the required reagents (excluding radioisotope) for performing transcription reactions in vitro and RQ1 RNase-Free DNase (Cat.# M6101) for removal of the template following transcription.

Features - Benefits

• Flexible: Allows synthesis of RNA corresponding to either the coding or noncoding strand of cloned DNA from a single plasmid construct.
• Specific: SP6, T7 and T3 RNA Polymerases are extremely promoter-specific, allowing production of virtually homogeneous RNA using plasmid DNA as a template.
• Convenient: Includes positive control template for use with T7, T3 or SP6 RNA polymerase, DNase I for removal of DNA template and Recombinant RNasin® Ribonuclease Inhibitor.

Applications

• Generation of high-specific-activity RNA probes.
• Generation of microgram quantities of RNA transcripts.
• Generation of RNA processing substrates.
• Generation of antisense RNA.

For more information, see the Protocols & Applications Guide.

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