See All pGL4 Vectors
Promoterless Renilla luciferase vectors are designed primarily to accept a putative promoter element for investigation of important regions controlling gene transcription. Alternatively, they may be used as promoterless control vectors in a dual-reporter system with a firefly luciferase vector serving as the experimental vector. The promoterless vectors are available with three varieties of engineered firefly luciferase genes: hRluc, hRlucP or hRlucCP. The hRluc gene is engineered to remove most cryptic transcription factor binding sites and improve mammalian expression through codon o...
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See All pGL4 Vectors
Promoterless Renilla luciferase vectors are designed primarily to accept a putative promoter element for investigation of important regions controlling gene transcription. Alternatively, they may be used as promoterless control vectors in a dual-reporter system with a firefly luciferase vector serving as the experimental vector. The promoterless vectors are available with three varieties of engineered firefly luciferase genes: hRluc, hRlucP or hRlucCP. The hRluc gene is engineered to remove most cryptic transcription factor binding sites and improve mammalian expression through codon optimization. The hRlucP and hRlucCP and RapidResponse™ genes are hRluc genes appended with degradation sequences to influence the cellular half-life of the hRluc gene. The RapidResponse™ genes respond more rapidly to stimuli but at the expense of signal intensity. The hRlucP gene responds more rapidly than hRluc2 with moderate signal intensity, and the hRlucCP responds more quickly with the lowest signal intensity. The promoterless vectors are available with or without selectable markers (hygromycin, neomycin or puromycin).
Features - Benefits
Improved Sensitivity and Biological Relevance Due to:
- Increased Reporter Gene Expression: Codon optimization of synthetic genes for mammalian expression.
- Reduced Background and Risk of Expression Artifacts: Removal of cryptic DNA regulatory elements and transcription factor binding sites.
- Improved Temporal Response: Rapid Response™ technology available using destabilized luciferase genes.
Additional Advantages Include:
- Flexible Detection Options: Choice of either synthetic luc2 (Photinus pyralis) or hRluc (Renilla reniformis) reporter genes.
- Easy Transition from Transient to Stable Cells: Choice of mammalian selectable markers.
- Easy Transfer from Vector to Vector: Common multiple cloning site and a unique SfiI transfer scheme.
Applications
- Transcription regulation.
- Virus-cell interactions.
- Compound screening.
- Post-translational modifications.
- GPCR signaling.
- Cell signaling.
- Promoter analysis.
For more information, see the Protocols & Applications Guide.
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