Dual-Glo® Luciferase Assay System

The Dual-Glo® Luciferase Assay System is a homogeneous reagent system that enables fast and simple quantitation of a stable luminescent signal from two reporter genes in a single sample. This convenient "add-and-read" system generates both firefly and Renilla luciferase luminescence signals from cells that have not been preconditioned or prelysed. The Dual-Glo® Luciferase Assay System provides high Z´-factors for cell-based, high-throughput screening applications. With the Dual-Glo® System, internal controls can be established to minimize sample variability by reducing false-positive and false-negati...

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The Dual-Glo® Luciferase Assay System is a homogeneous reagent system that enables fast and simple quantitation of a stable luminescent signal from two reporter genes in a single sample. This convenient "add-and-read" system generates both firefly and Renilla luciferase luminescence signals from cells that have not been preconditioned or prelysed. The Dual-Glo® Luciferase Assay System provides high Z´-factors for cell-based, high-throughput screening applications. With the Dual-Glo® System, internal controls can be established to minimize sample variability by reducing false-positive and false-negative readings caused by nonspecific factors such as cytotoxicity. In the Dual-Glo® Luciferase Assay, the activity of the primary reporter is correlated with the effect of specific stimuli, and the activity of the co-transfected control reporter provides an internal control to normalize results. The system is optimized for batch processing both 96- and 384-well plates and is compatible with a wide variety of mammalian cell culture media.

Features - Benefits

• Increased Precision and Accuracy: Normalize primary reporter results with an internal control, a co-reporter that minimizes effects of cell number and health, transfection efficiency and nonspecific cellular responses.
• Homogeneous Format: Perform fewer steps. Assay cells directly in growth medium for both reporters. No centrifugation or lysis steps required.
• Stable Signal: Obtain flexibility for either batch or continuous processing of 96- and 384-well plates. Each luminescent signal can be measured for up to 2 hours after reagent addition.
• Convenience: Screen efficiently with simple, two-step assay ideal for any luminometer. On-board injectors not required.
• Wide Dynamic Range: Analyze high and low reporter activity without sample dilution. Linear over at least 6 logs of enzyme concentration for each reporter.
• Choose Your Configuration: Learn more about our custom options for this product at: www.promega.com/myway/

Applications

For more information, see the Protocols & Applications Guide.

Notes

Please contact Promega for information on bulk purchases.

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