Promega Corporation

HisLink™ Protein Purification Systems

HisLink™ Protein Purification Systems are designed for purification of polyhistidine (His)-tagged or HQ-tagged proteins directly from culture medium containing bacterial cells expressing the tagged protein of interest. All product formats use the HisLink™ Protein Purification Resin (Cat.# V8821), a macroporous silica resin derivatized with a high level of tetradentate-chelated nickel (>20mmol Ni/ml settled resin). The resin performs well in either column, batch or vacuum-based methods with a binding capacity of >15mg/ml of resin. The HisLink™ Protein Purification Resin is useful in all general ...

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  • Prices valid for Australian customers only
Product Size Conc. Catalog # *List Price Order QTY Add to Cart

HisLink™ Spin Protein Purification System

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HisLink™ Spin Protein Purification System

 Components

  • Collection Tubes

    A130D1 x 25/pk
  • FastBreak™ Cell Lysis Reagent, 10X

    V857D1 x 5ml
  • HisLink™ Protein Purification Resin

    V882C1 x 5ml
  • HisLink™ Binding/Wash Buffer

    V981A1 x 50ml
  • HisLink™ Elution Buffer

    V982A1 x 10ml
  • Spin Column

    V995A1 x 25 each
  • DNase I (lyophilized)

    Z358B1 x 1 vial
25 reactions
- V1320 A$ 346.00 Add to cart

HisLink™ 96 Protein Purification System

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HisLink™ 96 Protein Purification System

 Components

  • HisLink™ 96 Filtration/Collection Plates

    V484A1 x 1 pack
  • Binding/Wash Buffer

    V851C1 x 110ml
  • Elution Buffer

    V852C1 x 25ml
  • FastBreak™ Cell Lysis Reagent, 10X

    V857A1 x 15ml
  • HisLink™ Protein Purification Resin

    V882B1 x 25ml
  • DNase I (lyophilized)

    Z358B1 x 1 vial
1 × 96
- V3680 A$ 477.00 Add to cart

HisLink™ 96 Protein Purification System

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HisLink™ 96 Protein Purification System

 Components

  • HisLink™ 96 Filtration/Collection Plates

    V484A5 x 1 pack
  • Binding/Wash Buffer

    V851D2 x 280ml
  • Elution Buffer

    V852D1 x 125ml
  • FastBreak™ Cell Lysis Reagent, 10X

    V857B1 x 60ml
  • HisLink™ Protein Purifiication Resin

    V882A2 x 50ml
  • DNase I (lyophilized)

    Z358A1 x 1 vial
5 × 96
- V3681 A$ 2,009.00 Add to cart

HisLink™ Protein Purification Resin

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HisLink™ Protein Purification Resin

 Components

  • HisLink™ Protein Purification Resin

    V882C1 x 5ml
5ml
- V8823 A$ 64.00 Add to cart

HisLink™ Protein Purification Resin

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HisLink™ Protein Purification Resin

 Components

  • HisLink™ Protein Purifiication Resin

    V882A1 x 50ml
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  • This product can be added to a

  • Helix Refrigerator

This product is available through the Promega Helix onsite stocking program in a +4°C Helix Refrigerator. The program offers numerous convenient solutions to meet your lab's needs. The Helix Refrigerator is 5 cubic ft.


Click here to learn more about the Helix Collection »


Already a Helix Customer?

Request to add this product to your Helix »


50ml
- V8821 A$ 477.00 Add to cart


Available Separately

PRODUCT SIZE CONC CATALOG # *List Price ORDER QTY. Add to Cart

FastBreak™ Cell Lysis Reagent, 10X

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FastBreak™ Cell Lysis Reagent, 10X

 Components

  • FastBreak™ Cell Lysis Reagent, 10X

    V857A1 x 15ml
15ml
- V8571 A$ 56.00 Add to cart

FastBreak™ Cell Lysis Reagent, 10X

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FastBreak™ Cell Lysis Reagent, 10X

 Components

  • FastBreak™ Cell Lysis Reagent, 10X

    V857B1 x 60ml
60ml
- V8572 A$ 155.00 Add to cart

FastBreak™ Cell Lysis Reagent, 10X

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FastBreak™ Cell Lysis Reagent, 10X

 Components

  • FastBreak™ Cell Lysis Reagent, 10X

    V857C1 x 100ml
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  • This product can be added to a

  • Helix Cabinet, Refrigerator

This product is available through the Promega Helix onsite stocking program in a room temperature cabinet or a +4°C Helix Refrigerator. The program offers numerous convenient solutions to meet your lab's needs.


Click here to learn more about the Helix Collection »


Already a Helix Customer?

Request to add this product to your Helix »


100ml
- V8573 A$ 241.00 Add to cart

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Storage Conditions

Store the systems and the resin at 4°C. Plates may be stored at 4°C or room temperature. Store Spin Columns, Collection Tubes and FastBreak™ Cell Lysis Reagent at room temperature. After reconstitution, store the DNase I in aliquots at –20°C.

For product intended use please see Patents & Disclaimers tab.

Overview of polyhistidine- or HQ-tagged protein purification using the HisLink Spin Protein Purification System.Figure 1. Overview of polyhistidine- or HQ-tagged protein purification using the HisLink™ Spin Protein Purification System.
Overview of polyhistidine-tagged protein purification using the HisLink 96 Protein Purification System.Figure 2. Overview of polyhistidine-tagged protein purification using the HisLink™ 96 Protein Purification System.
Vac-Man Laboratory Vacuum Manifold.Figure 3. Vac-Man® Laboratory Vacuum Manifold.

Use Restrictions

V1320, V3680, V3681, V8823, V8821, V8571, V8572, V8573 For Research Use Only. Not for Use in Diagnostic Procedures.

Patents - Disclaimers

V1320, V3680, V3681, V8823, V8821 U.S. Pat. Nos. 7,112,552 and 7,354,750.

V8571, V8572, V8573 Patent Pending.

V1320, V3680, V3681, V8571, V8572, V8573 This product is licensed for use under U.S. Pat. No. 6,174,704.

V1320, V3680, V3681, V8823, V8821, V8571, V8572, V8573 Certain applications of this product may be covered by patents issued to parties other than Promega and applicable in certain countries. Because purchase of this product does not include a license to perform any of these patented applications, users of this product may be required to obtain a patent license depending upon the particular application and country in which the product is used.

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