Promega Corporation

MagneGST™ Pull-Down System

The MagneGST™ Pull-Down System is designed for detection of protein interactions between GST-fusion proteins expressed in bacterial lysates and prey proteins expressed in the TnT® Systems. Prey protein synthesized in the TnT® Quick Coupled Transcription/Translation Reaction is captured using bait protein (GST-fusion protein) immobilized on MagneGST™ Particles. Nonspecifically bound proteins are then washed away, and the prey protein is analyzed. Prey proteins can be detected by incorporating radioactively labeled methionine in the TnT® Quick reaction, followed by SDS-PAGE and autoradiography or by incorporating th...

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MagneGST™ Pull-Down System

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MagneGST™ Pull-Down System

 Components

  • Methionine

    L118A1 x 50μl
  • RQ1 RNase-Free DNase

    M610A1 x 1,000u
  • Nuclease-Free Water

    P119A1 x 1,250μl
  • MagneGST™ Cell Lysis Reagent

    V481B1 x 60ml
  • MagneGST™ Glutathione Particles

    V861A1 x 4ml
  • MagneGST™ Binding/Wash Buffer

    V870B2 x 200ml
  • TnT® T7 Quick Master Mix

    V88711 x 8 × 200μl
80 reactions
- V8870 A$ 927.00 Add to cart


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Storage Conditions

Store the TnT® T7 Quick Master Mix and Methionine at –70°C. Store the RQ1 RNase-Free DNase at –20°C. Store the Nuclease-Free Water, MagneGST™ Glutathione Particles, MagneGST™ Binding/Wash Buffer and Cell Lysis Reagent at 4°C.

For product intended use please see Patents & Disclaimers tab.

Representation of pull-down assay using bacterial expression of bait protein and TNT® cell-free system for the expression of the prey protein.Figure 1. Representation of pull-down assay using bacterial expression of bait protein and TnT® cell-free system for the expression of the prey protein.

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Use Restrictions

V8870 For Research Use Only. Not for Use in Diagnostic Procedures.

Patents - Disclaimers

V8870 Patent Pending.

V8870 This product is licensed for use under U.S. Pat. No. 6,174,704.

V8870 Any use of the product for diagnostics requiring clearance or approval by the U.S. Food and Drug Administration or a foreign equivalent may require a license under Mayo Clinic U.S. Pat. No. 6,027,913.

V8870 Certain applications of this product may be covered by patents issued to parties other than Promega and applicable in certain countries. Because purchase of this product does not include a license to perform any of these patented applications, users of this product may be required to obtain a patent license depending upon the particular application and country in which the product is used.

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