Promega Corporation

PNGase F

PNGase F is a recombinant glycosidase cloned from Elizabethkingia miricola and overexpressed in E. coli. PNGase F has a molecular weight of 36kDa. PNGase F catalyzes the cleavage of N-linked oligosaccharides between the innermost GlcNAc and asparagine residues of high mannose, hybrid and complex oligosaccharides from N-linked glycoproteins (Figure 1). PNGase F will not remove oligosaccharides containing Alpha-(1,3)-linked core fucose commonly found on plant glycoproteins.

Unit Definition: One unit of PNGase F will catalyze the deglycosylation of 1 nanomole of denatured Ribonuclease B (RNase B) in one minute...

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PNGase F

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PNGase F

 Components

  • PNGase F

    V483A1 x 500 units
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  • Helix Refrigerator

This product is available through the Promega Helix onsite stocking program in a +4°C Helix Refrigerator. The program offers numerous convenient solutions to meet your lab's needs. The Helix Refrigerator is 5 cubic ft.


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500 units
10u/μl V4831 A$ 177.00 Add to cart


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Storage Conditions

Store at 2–10°C.

For product intended use please see Patents & Disclaimers tab.

11123MAFigure 1. Cleavage specificity of PNGase F on N-Glycans.

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Recombinant PNGase F deglycosylates multiple substrates.Figure 2. Recombinant PNGase F deglycosylates multiple substrates.

Twenty micrograms of various denatured glycoprotein standards were treated with 10 units of Promega recombinant or native PNGase F for 1 hour at 37°C. Gel shifts indicating deglycosylation were monitored by SDS-PAGE.

Twenty micrograms of various denatured glycoprotein standards were treated with 10 units of Promega recombinant or native PNGase F for 1 hour at 37°C. Gel shifts indicating deglycosylation were monitored by SDS-PAGE.

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Schematic illustrating the use of PNGase F and mass spec analysis of N-glycosylation.Figure 3. Schematic illustrating the use of PNGase F and mass spec analysis of N-glycosylation.

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Use Restrictions

V4831 For Research Use Only. Not for Use in Diagnostic Procedures.

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