GoTaq® qPCR and RT-qPCR Systems for
Probe-based Detection

The GoTaq® Probe qPCR and RT-qPCR Systems are ready-to-use 2X master mixes that simplify the preparation of reactions for qPCR using hydrolysis probe detection. In combination with GoScript™ Reverse Transcriptase, one-step and two-step RT-qPCR systems are also available. The master mix employs rapid hot-start activation and processive enzymes, making it compatible with any real-time PCR instrument using standard or FAST cycling programs.

• Superior Performance
  Sensitive detection on any real-time instrument.
• Enhanced Stability
  Exceptional room-temperature setup makes it suitable for automation and high-throughput detection.
• Versatile
  Compatible with both FAST and standard cycling methods.
• Robust
  High-efficiency, full-length cDNA synthesis in the presence of inhibitors.
• Confidence
  The Promega PCR Performance Guarantee.

GoTaq® Probe 2-Step RT-qPCR System. cDNA was generated from 100ng of human pancreas total RNA, using the GoScript™ Reverse Transcription System. The human insulin gene (INS) was detected from five-fold serial dilutions of cDNA (100ng to 6.4pg), using GoTaq® Probe qPCR Master Mix for amplification. The resulting standard curve is shown in the inset (R² = 0.998, slope = –3.33).