Promega Corporation

AMV Reverse Transcriptase

Avian Myeloblastosis Virus Reverse Transcriptase (AMV RT) catalyzes the polymerization of DNA using template DNA, RNA or RNA:DNA hybrids. It requires a primer (DNA primers are more efficient than RNA primers) as well as Mg2+ or Mn2+. The enzyme possesses an intrinsic RNase H activity. Both nonionic detergents and sulfhydryl compounds stabilize the enzyme activity in vitro.

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  • Prices valid for Australian customers only
Product Size Conc. Catalog # *List Price Order QTY Add to Cart

AMV Reverse Transcriptase

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AMV Reverse Transcriptase

 Components

  • AMV Reverse Transcriptase

    M510A1 x 300u
  • AMV RT 5X Buffer

    M515A1 x 500μl
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  • Helix Freezer

This product is available through the Promega Helix onsite stocking program in a –20°C Helix Freezer. The program offers numerous convenient solutions to meet your lab's needs. Helix Freezers are available in two sizes: 5.7 cubic ft. or 9.7 cubic ft.


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Request to add this product to your Helix »


300u
10u/μl M5101 A$ 111.00 Add to cart

AMV Reverse Transcriptase

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AMV Reverse Transcriptase

 Components

  • AMV Reverse Transcriptase

    M510F1 x 1,000u
  • AMV RT 5X Buffer

    M515A3 x 500μl
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  • This product can be added to a

  • Helix Freezer

This product is available through the Promega Helix onsite stocking program in a –20°C Helix Freezer. The program offers numerous convenient solutions to meet your lab's needs. Helix Freezers are available in two sizes: 5.7 cubic ft. or 9.7 cubic ft.


Click here to learn more about the Helix Collection »


Already a Helix Customer?

Request to add this product to your Helix »


1,000u
10u/μl M5108 A$ 293.00 Add to cart

AMV Reverse Transcriptase (HC)

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AMV Reverse Transcriptase (HC)

 Components

  • AMV RT 5X Buffer

    M515A1 x 500μl
  • AMV Reverse Transcriptase (HC)

    M900A1 x 600u
Close Window
  • This product can be added to a

  • Helix Freezer

This product is available through the Promega Helix onsite stocking program in a –20°C Helix Freezer. The program offers numerous convenient solutions to meet your lab's needs. Helix Freezers are available in two sizes: 5.7 cubic ft. or 9.7 cubic ft.


Click here to learn more about the Helix Collection »


Already a Helix Customer?

Request to add this product to your Helix »


600u
20–25u/μl M9004 A$ 223.00 Add to cart


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QC Tests

Activity, SDS-PAGE/purity, DNase, RNase, endonuclease, RT-PCR, first-strand cDNA synthesis.

Source

Purified Avian Myeloblastosis Virus particles.

Storage Buffer

200mM potassium phosphate (pH 7.2 at 4°C), 0.2% Triton® X-100, 2mM DTT and 50% gycerol.

Storage Conditions

Store at –20°C.

Unit Definition

One unit is defined as the amount of enzyme required to catalyze the transfer of 1nmol of dTTP into acid-insoluble form in 10 minutes at 37°C. The reaction conditions are: 50mM Tris-HCl (pH 8.3 at 25°C), 40mM KCl, 8.75mM MgCl2, 10mM DTT, 0.1mg/ml acetylated BSA, 1mM radiolabeled dTTP and 0.25mM poly(A):oligo(dT).

For product intended use please see Patents & Disclaimers tab.

Use Restrictions

M5101, M5108, M9004 For Laboratory Use. Outside of the United States, this product is intended for research use only unless otherwise stated.

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