Anti-Human NT-3 pAb

Neurotrophin-3, a 27kDa homodimer that shares high sequence homology with NGF, BDNF, NT-4 and NT-5, influences many neuron types in the central and peripheral nervous system. NT-3 is also highly conserved across species. Anti-Human NT-3 pAb is generated in chickens and purified using a proprietary polyethylene glycol procedure. IgY, the 180kDa chicken IgG homolog, can be produced in chickens against certain biological antigens that fail to elicit a humoral immune response in rabbits or other mammals due to species relatedness. This antibody is highly specific for NT-3 in a variety of mammalian species.

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Neurotrophin-3, a 27kDa homodimer that shares high sequence homology with NGF, BDNF, NT-4 and NT-5, influences many neuron types in the central and peripheral nervous system. NT-3 is also highly conserved across species. Anti-Human NT-3 pAb is generated in chickens and purified using a proprietary polyethylene glycol procedure. IgY, the 180kDa chicken IgG homolog, can be produced in chickens against certain biological antigens that fail to elicit a humoral immune response in rabbits or other mammals due to species relatedness. This antibody is highly specific for NT-3 in a variety of mammalian species.

Features - Benefits

• Immunogen: Human recombinant NT-3.
• Antibody Form: Chicken IgY is provided at 0.5mg/ml in 0.1M NaCl, 0.01M K₂HPO₄ and 50μg/ml gentamicin.
• Specificity: Cross-reactive with human and mouse NT-3 and is presumed to cross-react with rat and Rhesus monkey NT-3 based on factor sequence identity across species; does not cross-react with BDNF or NGF and has limited cross-reactivity to NT-4.

Applications

(Suggested working concentration)

• Western blotting (1μg/ml).
• ELISA (1μg/ml).
• Immunocytochemistry (1–10μg/ml).
• Biological neutralization (1–10μg/ml).

For more information, see the Protocols & Applications Guide.

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