GDNF E_max® ImmunoAssay Systems

The GDNF E_max® ImmunoAssay Systems provide optimized reagents and a protocol for the sensitive and specific detection of glial cell-line-derived neurotrophic factor (GDNF). After an overnight coating of a 96-well plate, the specific protein is detected using an antibody sandwich format. The systems use horseradish peroxidase-conjugated secondary antibody and a single-component TMB substrate for the final chromogenic detection of bound neurotrophic factor. Using this system, GDNF in tissue culture supernatants or tissue homogenates can be quantitated in the range of 15.6–1,000pg/ml.

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The GDNF E_max® ImmunoAssay Systems provide optimized reagents and a protocol for the sensitive and specific detection of glial cell-line-derived neurotrophic factor (GDNF). After an overnight coating of a 96-well plate, the specific protein is detected using an antibody sandwich format. The systems use horseradish peroxidase-conjugated secondary antibody and a single-component TMB substrate for the final chromogenic detection of bound neurotrophic factor. Using this system, GDNF in tissue culture supernatants or tissue homogenates can be quantitated in the range of 15.6–1,000pg/ml.

Features - Benefits

• High Value: Optimized reagents and protocol provided.
• Specificity: Specific detection of GDNF; less than 3% cross-reactivity with other related neurotrophic and growth factors.
• Sensitivity: Detect picogram levels of factor per milliliter of sample.
• Flexibility: Available kits provide sufficient reagents for two or five 96-well plates; you can configure your plates as desired.

Plates are not included.

References

1. Okragly, A.J. and Haak-Frendscho, M. (1997) Exp. Neurol. 145, 592–6.

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