PureYield™ Plasmid Miniprep System

Special Offer: Switch to the Vacuum Format and get a Free Welch® Vacuum Pump! For a limited time PureYield™ Plasmid Miniprep Start-Up Kits are available. Purchase a PureYield™ Plasmid Miniprep System and a Vac-Man® Vacuum Manifold, and get a free Welch® Vacuum Pump! Each vacuum pump bundle is available in 3 formats. If you are not sure which format is right for your geographic area, please contact your Promega representative or an authorized Promega distributor.

The PureYield™ Plasmid Miniprep System is designed to rapidly isolate highly pure plasmid DNA. The system provides a rapid method for purificat...

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Special Offer: Switch to the Vacuum Format and get a Free Welch® Vacuum Pump! For a limited time PureYield™ Plasmid Miniprep Start-Up Kits are available. Purchase a PureYield™ Plasmid Miniprep System and a Vac-Man® Vacuum Manifold, and get a free Welch® Vacuum Pump! Each vacuum pump bundle is available in 3 formats. If you are not sure which format is right for your geographic area, please contact your Promega representative or an authorized Promega distributor.

The PureYield™ Plasmid Miniprep System is designed to rapidly isolate highly pure plasmid DNA. The system provides a rapid method for purification of up to 15μg of plasmid DNA from 600μl to 3ml of bacteria culture. Plasmid DNA can be purified in as little as 10 minutes. The PureYield™ Plasmid Miniprep System incorporates a unique Endotoxin Removal Wash designed to remove substantial amounts of protein, RNA and endotoxin contaminants from purified plasmid DNA. Removal of contaminants improves the robustness of sensitive applications such as eukaryotic transfection, in vitro transcription and coupled in vitro transcription/translation (e.g., TnT® Quick Coupled Transcription/Translation System). Purification is achieved without isopropanol precipitation of purified plasmid DNA or extensive centrifugation, providing rapid purification from a single method.

The system has been designed for use with centrifugation or vacuum (e.g., the Vac-Man® Laboratory Vacuum Manifold).

Features - Benefits

• Improved Productivity: Rapid protocol purifies plasmid DNA in 10 minutes.
• Robust Performance: High purity and concentration of plasmid DNA gives proven performance in transfection, cell-free expression and other molecular biology applications.
• Confidence in Results: Lysis/neutralization indicator dye ensures success every time.
• Flexible: Centrifugation and vacuum protocols are available.

Applications

For more information, see the Protocols & Applications Guide.

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