pSP72 Vector

The pSP72 Vector can be used as a standard cloning vector and also can be used for transcription of RNA in vitro. The pSP72 Vector contains the SP6 and T7 RNA polymerase promoters flanking a unique multiple cloning region, which includes restriction sites for XhoI, PvuII, HindIII, SphI, PstI, SalI, AccI, XbaI, BamHI, SmaI, KpnI, SacI, EcoRI, ClaI, EcoRV and BglII. The pSP72 and pSP73 Vectors are essentially identical except for the orientation of the multiple cloning site region.

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The pSP72 Vector can be used as a standard cloning vector and also can be used for transcription of RNA in vitro. The pSP72 Vector contains the SP6 and T7 RNA polymerase promoters flanking a unique multiple cloning region, which includes restriction sites for XhoI, PvuII, HindIII, SphI, PstI, SalI, AccI, XbaI, BamHI, SmaI, KpnI, SacI, EcoRI, ClaI, EcoRV and BglII. The pSP72 and pSP73 Vectors are essentially identical except for the orientation of the multiple cloning site region.

Features - Benefits

• Versatile: This vector can be used for standard cloning and in vitro transcription from SP6 and T7 RNA polymerase promoters flanking the multiple cloning region.
• Convenient: Multiple cloning site provides a selection of restriction sites for cloning.

References

1. Krieg, P.A. and Melton, D.A. (1987) Meth. Enzymol. 155, 397–415.

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