Pro 5-alpha can be used for the efficient transformation of unmethylated DNA derived from PCR, cDNA and many other sources (hsdR). The elimination of nonspecific endonuclease I (endA1) enables the highest quality plasmid preparations. The strain is resistant to phage T1 (fhuA2) and suitable for blue/white screening by α-complementation of the β-galactosidase gene. Single-use Pro 5-alpha Competent Cells are available in high efficiency at greater than 109cfu/µg.
JM109 and HB101 Competent Cells are prepared according to a modified procedure of Hanahan.
JM109 is a K strain that is recA– and ...
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Pro 5-alpha can be used for the efficient transformation of unmethylated DNA derived from PCR, cDNA and many other sources (hsdR). The elimination of nonspecific endonuclease I (endA1) enables the highest quality plasmid preparations. The strain is resistant to phage T1 (fhuA2) and suitable for blue/white screening by α-complementation of the β-galactosidase gene. Single-use Pro 5-alpha Competent Cells are available in high efficiency at greater than 109cfu/µg.
JM109 and HB101 Competent Cells are prepared according to a modified procedure of Hanahan.
JM109 is a K strain that is recA– and endA– to minimize recombination and improve the quality of plasmid DNA. In addition, the cells carry the F´ episome, which allows blue/white screening. JM109 Competent Cells are available for convenient transformation in two efficiencies: High Efficiency at greater than 108cfu/μg and Subcloning Efficiency at greater than 107cfu/μg.
HB101 cells are recA–, which minimizes recombination events, and contain a restriction-minus background (hsdS20) that allows better representation when cloning methylated DNA. HB101 Competent Cells are suitable for vectors that do not require α-complementation for blue/white screening.
For researchers doing more than one transformation, competent cells are available in standard format (200µl aliquots). For added convenience, single-use competent cells (50µl aliquots) also are offered.
Pro 5-alpha Genotype: fhuA2, Δ(argF-lacZ), U169, phoA, glnV44, φ80, Δ(lacZ)M15, gyrA96, recA1, relA1, endA1, thi-1, hsdR17.
JM109 Genotype: endA1, recA1, gyrA96, thi, hsdR17 (rk–, mk+), relA1, supE44, Δ( lac-proAB), [F´ traD36, proAB, laqIqZΔM15].
HB101 Genotype: F–, thi-1, hsdS20 (rB–, mB–), supE44, recA13, ara-14, leuB6, proA2, lacY1, galK2, rpsL20 (strr), xyl-5, mtl-1.
Features - Benefits
- Convenient: Ready to use; no preparation time necessary.
- Reliable: Transformation efficiencies guaranteed.
- Safe: The recA– mutation prevents undesirable recombination events, and the endA– mutation in JM109 cells prevents carryover nuclease in miniprep DNA.
Applications
For more information, see the Protocols & Applications Guide.
References
- Hanahan, D. (1985) DNA Cloning, Vol. 1, Glover, D., ed., IRL Press, Ltd., 109.
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