Luminogenic Enzyme Substrates

The pro-luciferin substrates can be used to monitor the activity of specific isoforms of cytochrome P450 or NAT2 as indicated in the name of the substrate. The Luciferin-MultiCYP is a promiscuous substrate that reacts with at least 21 P450 isoforms and is useful for measuring net CYP activity in a mixed population of P450s. The Luciferin-NAT2 is an excellent substrate for N-acetyltransferase 2 (NAT2), a phase II biotransformation enzyme that acetylates aromatic amine groups on xenobiotic compounds. This substrate shows little to no cross-reactivity with NAT1.

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The pro-luciferin substrates can be used to monitor the activity of specific isoforms of cytochrome P450 or NAT2 as indicated in the name of the substrate. The Luciferin-MultiCYP is a promiscuous substrate that reacts with at least 21 P450 isoforms and is useful for measuring net CYP activity in a mixed population of P450s. The Luciferin-NAT2 is an excellent substrate for N-acetyltransferase 2 (NAT2), a phase II biotransformation enzyme that acetylates aromatic amine groups on xenobiotic compounds. This substrate shows little to no cross-reactivity with NAT1.

Notes

Luciferin Detection Reagent must be used in combination with the pro-luciferin substrates on this page. Use V8921 with P1721, P1741, P1621, P1651 and P1661. V8931 must be used with P1671 and P1731, as these substrates are ester derivatives.

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